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Biotechnology Series Vol 1 : Animal Cell Culture
Editor: B.P. Mishra, B. Mishra, P. P. Bhat and P.N. Bhat
Price: Rs.: 195/-
ISBN: 978-93-80012-50-6
Pages: 128
Year: 2011
Publisher: Studium Press (India) Pvt. Ltd.
Readership: Biotechnology, Veterinary Science


 
Book Contents:
Introduction, Historical development, Land mark achievements, Tissue culture, Cell culture, Organ culture, Cells in culture, The characteristics of animal cells in culture, Behaviour of cells in culture, Differentiation; Lab design, equipments and materials, Design and layout, Construction and services, Layout of aseptic room, Sterile handling area, Incubation, Preparation area, Wash-up, Sterilization, Storage, Equipments, Laboratory operation; Sterilization procedures, Introduction, Principles of sterilization, Methods of sterilization, Hot air ovens, Steam sterilization, Physical removal: filtration, Radiation, Chemical sterilization, Selection of detergent, Chemicals used in sterilization, Sterilization of tissue culture glass wares, Sterilization of tissue culture reagents, Sterilization of media, Quality control of sterilization; Culture media and reagents, Introduction, Purpose of media, Types of media, Natural media, Artificial media, Selection of media, Ingredients of media/basic composition of tissues, Components of media, Preparation of media, Balanced salt solutions, Synthetic or defined media, Preparation of media for sterilization, Tissue culture reagents, L-Glutamine, Antibiotics, HEPES/bicarbonate solution, Biological buffers for cell culture medium; Preparation of sera, Introduction, What is serum?, Role of serum in cell culture, Role of serum components, Serum components, Growth factors, Hormones, Attachment and spreading factors, Binding proteins, Lipids, Minerals, Advantages of serum in tissue culture, Disadvantages of using serum in tissue cultu, Requirement of serum free media, Serum-free cell culture, Content of serum-free media, Preparation of serum-free media, Commercially available serum-free media, Potential advantages of using low serum and serum free media, General procedure for preparation of sera, Preparation of chicken serum, Preparation of mammalian serum, Sterilization, Storage, Serum thawing and inactivation, Quality control, Cell plating efficiency assay, Cloning efficiency, Growth curve, Batch reservation, Contamination of serum, Protein-free media, Chemically-defined media; Primary and suspension cultures, Introduction, Source of material, Adult or embryonic tissue, Adherent or suspension culture, Primary cultures or continuous cell lines, Primary cultures: Advantages and limitations, Continuous (Clonal) cell lines: Advantages and limitions, Selection of particular cell type, Collection and processing of tissue, Steps followed in establishing a culture, Checking and prevention for contamination, Procedure for primary culture, Materials and techniques, Isolation of cells from blood and other fluids (general), Isolation of chicken embryo fibroblasts (CEF), Separation of mononuclear cells from human/animal, peripheral blood, Animal cells, Preparation of murine spleen cells, Mouse embryo cell culture, Lamb testis cell culture; Cell counting and vital staining, Introduction, Packed cell volume, Haemocytometer counting, Electronic particle counting, Trypan blue exclusion assay, Neutral red staining, Neutral red uptake, In vitro limitations; Growth kinetics, Introduction, Absolute growth rate (AGR), Relative growth rate (RGR), Cell cycle and cell differentiation, Determination of doubling time, Cell differentiation, Cell cycle, Labelled mitoses method, Plating efficiency; Culture parameters, Introduction , pH, CO2 and bicarbonate, Buffering, Oxygen, Cell density; Chromosome karyotyping, Introduction, Staining, Preparation of karyotype; Cell lines, Introduction, Cell line, Establishment of primary cultures from various sources, Culturing continuous cell lines; Types of culture systems, Batch culture, Scale-up in suspension, Continuous culture, Adchorage dependent cells, Scale and complexity, Mixing and aeration, Air-lift fermentors, Scale-up of monolayer cultures, Multisurface arrangement, Roller culture, Protocol 12.1 roller bottle culture, Protocol details, Analysis, Scaling up of large capacity stationary cultures, Perfused monolayer culture, Fixed-bed reactors, Fluidized-bed reactors for monolayer cultures Monitoring and growth in bulk cultures; Preservation characterization storage and transport of cells, Characterization of cell lines, Species verification, Cytogenetics analysis, Karyotype techniques, Characterization of cell type and stage of differentiation, Verifying tissue of origin, Connective tissue, Epithelial tissue, Muscular tissue, Nervos tissue, Blood and lymph tissue, Storage of cells in frozen state, Cryopreservatives, Freezing mixture, Freezing down cells, Revival of frozen cells and quantization, Transport of cell cultures and cell suspensions, Managing Cell Cultures, Obtaining the basic material: Importance of cell culture, collections, Cell culture databases, Managing cell culture, Laboratory design, Handling of cell lines; Cell lines bank, Introduction, Cell of cell line banking; Flow cytometry, Introduction, Applications of flow cytometry, Reference, Suggested reading, Glossary, Subject index
 
About The Book:
India is likely to become a major force in the area of Biotechnology in the next decade. The Department of Biotechnology has encouraged human resource development by creating new institutions and centres of excellence for the research opportunities in this directions. Any research work in biotechnology uses cell culture as a basic tool in manipulation of cell organelle genetically as well as phenotypically. This book is an attempt to comprehensively present the information on animal cell and tissue culture in single volume. It is intended primarily as a text book for undergraduate and post graduate students majoring in Biotechnology, Biosciences, Biotechnology product formulations, preparation of vaccines and diagnostic used in veterinary and human medicine. The new biology is being inducted through animal cell culture in to animal industry. This book has fifteen chapters which deal with historic developments, laboratory design, sterilization procedures and various facets of animal cell culture. This includes preservation, characterizations, storage and transport of cells, their monitoring and technologies for cell banking.
 
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